Recombinant Mouse IL-7 Protein (C-6His)
产品特性
| Synonyms: | IL-7; IL-7 interleukin-7; interleukin-7; Lymphopoietin -1; PBGF |
| Product Description: | Recombinant Mouse Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Glu26-Ile154 is expressed with a 6His tag at the C-terminus. |
| UniProt ID: | P10168 |
| Species: | Mouse |
| Expression System: | Human Cells |
| Tag: | C-6His |
| Predicted MW: | 15.9 KDa |
| SDS-PAGE: | 22-28 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Endotoxin Level: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Activity: | Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 60-1000 pg/ml. |
| Loaded Mouse IL-7RA-Fc on Protein A Biosensor, can bind Mouse IL-7-His with an affinity constant of 18.9 nM as determined in BLI assay. | |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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