Recombinant Human VEGF165 Protein
产品特性
| Synonyms: | Vascular Endothelial Growth Factor Isoform 165; VEGF165 |
| Product Description: | Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed. |
| UniProt ID: | P15692-4 |
| Species: | Human |
| Expression System: | Human Cells |
| Tag: | Tag free |
| Predicted MW: | 19.1 KDa |
| SDS-PAGE: | 18-22 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Endotoxin Level: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Activity: | Immobilized Human VEGF165 (Cat#CK-18-2101) at 2μg/ml (100 μl/well) can bind Human VEGF165 antibody. The ED50 of Human VEGF165 antibody is 1.44 ng/ml. |
| Loaded Human VEGFR1-Fc on Protein A Biosensor, can bind Human VEGF165(Cat#CK-18-2101) with an affinity constant of 0.29 nM as determined in BLI assay. | |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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