Recombinant Human RSPO1 Protein (C-6His)
产品特性
| Synonyms: | RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof plate-specific spondin-1 |
| Product Description: | Recombinant Human R-spondin-1 is produced by our Mammalian expression system and the target gene encoding Ser21-Ala263 is expressed with a 6His tag at the C-terminus. |
| UniProt ID: | Q2MKA7 |
| Species: | Human |
| Expression System: | Human Cells |
| Tag: | C-6His |
| Predicted MW: | 27.8 KDa |
| SDS-PAGE: | 40 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Endotoxin Level: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Activity: | Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4.06 ng/ml. |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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