Anti-His tag, Recombinant VHH (HRP)
Specifications
| Product Cat#: | AB-06-4810 |
| Product type: | Primary antibody |
| Antigen: | His tag |
| Immunogen: | His tag |
| Species immunized: | Alpaca |
| Applications: | Western Blot (1:2000-1:10000); ELISA (1:5000-1:20000) |
| Reactivity: | C-terminus site of His tag fusion protein. |
| Clonality (clone number): | Monoclonal |
| Form: | Liquid |
| Buffer: | 1*PBS (pH7.4), 10 mg/ml BSA, 50% glycerol and 100mM trehalose. |
| Concentration: | 1 mg/ml |
| Purity: | Immunogen affinity purified. |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Conjugate: | HRP |
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Target information
C-His-tags are a series of consecutive histidine residues, six, which are typically fused to the N- or C-terminal end of recom binant proteins. Its small size minimizes interference with the structure and folding of the protein. C-His-tag residues bind to several transition metalions such as nickel, cobalt, and copperwhich, when immobilized on beads or a resin, allows for the simple purification of His-tagged proteins. The antibody is useful for the detection of C-terminal His-tag residues of 6 or more in WB,IP, ELISA / FLISA, flow cytometry,and immunochemistry applications. Directly conjugated HRP allow for detection without the use of a secondary antibody.
Provider
Erpantech Laboratory