Anti-6×His tag, Recombinant VHH
Specifications
| Product Cat#: | AB-06-5062 |
| Product type: | Primary antibody |
| Antigen: | 6×His tag |
| Immunogen: | His tag (HHHHHH) |
| Molecular Weight: | 15.57 KD |
| Isotype: | VHH domain of alpaca IgG2a/2b |
| Species immunized: | Alpaca |
| Reactivity: | Recognizes the His-tag sequence motif HHHHHH at the N-terminus, C-terminus, or internal site of the fusion protein. |
| Form: | Liquid |
| Buffer: | 0.1M Na2CO3 (PH 9.6) |
| Concentration: | 1 mg/ml |
| Purity: | Recombinant Expression and Affinity purified by HA Nanoselector Agagrose. |
| Clones Affinity (KD): | 0.32 nM |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Conjugate: | Unconjugated |
Target information
His-tags are a series of consecutive histidine residues, commonly six, which are typically fused to the N- or C-terminal end of recombinant proteins. Its small size minimizes interference with the structure and folding of the protein. His-tag residues bind to several transition metal ions such as nickel, cobalt, and copper which, when immobilized on beads or a resin, allows for the simple purification of His-tagged proteins. The antibody is useful for the detection of N- and C-terminal as well as exposed internal His-tag residues of 6 or more in western blotting, immunoprecipitation, ELISA / FLISA, flow cytometry, and immunocytochemistry applications. Directly conjugated options allow for detection without the use of a secondary antibody.
Provider
Erpantech Laboratory