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Rabbit anti Rat IgG (H+L)-HRP antibody

SKU Product Brand Unit Availability Price Quantity  
AB-06-4771
Rabbit anti Rat IgG (H+L)-HRP antibody
Erpan Tech In stock

Specifications        

Product Cat#: AB-06-4771
Product type: Secondary antibody
Antigen: Rat IgG (H+L)
Immunogen: Rat IgG (H+L)
Species immunized: Rabbit
Isotype: IgG
Applications: Western Blot (1:5000-1:10000); ELISA (1:10000)
Reactivity: Rat
Clonality (clone number): Polyclonal
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol.
Concentration: 2 mg/ml
Purity: Protein affinity purified.
Storage: Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles.
Conjugate: HRP-conjugated
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Target information

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Provider

Erpantech Laboratory