Anti-6X His-Tag antibody
Specifications
| Product Cat#: | AB-06-0102 |
| Product type: | Primary antibody |
| Antigen: | 6X His-Tag |
| Immunogen: | Synthetic peptide immune sequence is CHHHHHHHH. |
| Species immunized: | Mouse |
| Isotype: | IgG2a |
| Applications: | Western Blot (1:2000); Immunoprecipitation (2-5 µg/ml) |
| Clonality (clone number): | Monoclonal (8-F0) |
| Form: | Liquid |
| Buffer: | Tris-HCl buffer (pH7.4), 0.2% BSA, 50% glycerol. |
| Concentration: | 2 mg/ml |
| Purity: | Protein G affinity purified. |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | 6 His epitope tag antibody |
| Hexa His tag antibody | |
| HHHHHH epitope tag antibody | |
| HHHHHH tag antibody | |
| His tag antibody |
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antigen information
A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6xHis tag.
Provider
Erpantech Laboratory